Fig. 4. Adsorption of his-tagged DspB on Ni-MNPs. Panel (A), SDS-PAGE analysis, Lanes B and A show cell lysate containing his-tagged DspB before and after adsorption on Ni-MNPs; Lanes W and E show wash and elution fractions. Green arrow points to the position of his-tagged DspB. Panel (B), elution of DspB under different concentrations of imidazole (Fig. S1, see ESI†). Panel (C), adsorption of DspB and pseudo-second-order adsorption kinetic plots (inset). Panel (D), dissociation of DspB and Langmuir isotherm fitting (inset).