Kinetic parameters of free and immobilized DspB.
DspB samples | K m (mM) | V max (mM min−1) |
---|---|---|
Before immobilization | 48.7 | 0.13 |
Ni-FS-TED | 21.6 | 0.04 |
Ni-FS-IDA | 32.7 | 0.20 |
Ni-FS-ECH-IDA | 34.1 | 0.30 |
DspB samples | K m (mM) | V max (mM min−1) |
---|---|---|
Before immobilization | 48.7 | 0.13 |
Ni-FS-TED | 21.6 | 0.04 |
Ni-FS-IDA | 32.7 | 0.20 |
Ni-FS-ECH-IDA | 34.1 | 0.30 |