Fig. 2. a) Diagrammatic representation for optimization of aptamer concentration for the detection of aflatoxin B1; (b) absorption spectra of different concentration of aptamer (800, 400, 200, 100, 50, 25 nM); (c) calibration curve for detection of optimum concentration of aptamer (25, 50, 100, 200, 400, 800 nM) and correspondence color change in the presence of different concentration of aptamer; (d) zeta potential of different concentration of aflatoxin B1 aptamer; (e) hydrodynamic diameter of AuNPs, AuNPs + AflB1 Apt, AuNPs + NaCl, and AuNPs–AflB1 + NaCl nanocomplex.
