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. 2021 Nov 19;145(3):879–886. doi: 10.1093/brain/awab373

Figure 1.

Figure 1

Medial forebrain bundle 6-OHDA increases the fraction of DA neurons expressing VGLUT2 in both SNc and VTA. (A) Schematic of unilateral medial forebrain bundle (MFB) 6-OHDA injection leading to anterograde and retrograde degeneration of midbrain DA neurons. (B) Cartoon depicting selective 6-OHDA (red circles) uptake via the DA transporter (DAT, green) into DA varicosities where it induces rapid degeneration through oxidative stress and mitochondrial disruption. (C) Example images showing coronal sections with TH+ midbrain DA neurons from vehicle- and 6-OHDA-injected animals. Note reduction in TH mRNA signal in both VTA (blue arrows) and SNc (white arrows) of 6-OHDA treated mouse (right panel). (D) DA neuron counts are reduced, but the fraction of DA neurons that express VGLUT2 is increased in SNc and (E) in VTA; **P < 0.01; ***P < 0.001, two-tailed unpaired t-test.