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. 2022 Apr 28;3(4):471–485. doi: 10.1038/s43018-022-00355-4

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 7 — For all samples, an initial manual gate in SSC-A by FSC-A was set to identify live cells and exclude debris. From the live cells, a rectangular gate was set on FSC-H by FSC-A to exclude doublets, meaning cells off the diagonal. If DNA staining was carried out, an additional gate was set on DNA (Violet channel)-W by DNA-A to exclude additional cell debris or DNA doublets.