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. 2022 Apr 28;12:6913. doi: 10.1038/s41598-022-10688-w

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Total protein extract obtained from the subcutaneous adipose tissue was used for Western Blotting experiments. Uncut images can be viewed in the supplementary material. The membranes were cut at the specific height of the target protein. (A) The protein content of (B) PPARγ, (C) PGC-1α, (D) PRDM16, and (E) UCP1. The bars were relativized by the CT group (100%). In browning pathway, the signal of statistical difference are represented as follows *p < 0.05 CT versus OBS, **p < 0.05 CT versus OBexT and ***p < 0.05 OBS versus OBexT (n = 4 CT group; n = 5 OBS group; n = 5 OBexT group). In (F), the effect of strength training protocols on the induction of mRNA expression of genes involved in browning subcutaneous adipose tissue. Representative analysis in control, obese sedentary, and obese training swiss animals. Gene expression analysis of Pparγ, Pgc-1α, Prdm16, and Ucp1. In browning gene, the signal of statistical difference are represented as follows *p < 0.05 CT versus OBS, **p < 0.05 CT versus OBexT and ***p < 0.05 OBS versus OBexT (n = 7 CT group; n = 4 OBS group; n = 4 OBexT group). One-way Anova statistical analysis was used, followed by Tukey's post-test.