Fig. 6. Glucose-stabilizing activity of 2c on db/db mice and acute toxicity assay of 2c on normal Kunming mice. Glucose-stabilizing profiles of lixisenatide, semaglutide and 2c in db/db mice fasted for 16 h, after the administrations (i.p.) of these agents at 4 h (A) and 8 h (B) before OGTT. (C) Hypoglycemic activities of lixisenatide, semaglutide and 2c on non-fasted db/db mice. Biochemical analysis results of 2c on AST (D), ALT (E), BUN (F) and SCr (G) after two weeks dosing on normal Kunming mice. Means ± SD, n = 6. ^***P < 0.001 vs. saline, ^cP < 0.001 vs. lixisenatide. The immunogenicity testing of 2c was evaluated by in vitro T cell proliferation assay and compared with lixisenatide and semaglutide (H). The frequency of response (%) that showed a positive T-cell proliferative response over the course of the entire study (n = 50).