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. 2022 Apr 28;13:2297. doi: 10.1038/s41467-022-29751-1

Fig. 1. Teneurin-3 (Tenm3) is a synaptic protein that is present in discrete areas of the hippocampal formation.

Fig. 1

a Overall localization of Tenm3 in the hippocampal formation. Horizontal brain sections from a juvenile wild-type mouse were stained with antibodies to Tenm3 (green) and DAPI (blue). Left panel, overview; right four panels, higher magnification views of the proximal CA1, distal subiculum, medial entorhinal cortex (MEC), and dentate gyrus as indicated. Arrows point to areas of intense labeling, such as the S. lacunosum-moleculare of the proximal CA1, the molecular layer of distal subiculum, and the dentate gyrus. Asterisks represent the cell body of both proximal CA1 and distal subiculum. b Tenm3 is co-localized with excitatory synapse markers in the S. lacunosum-moleculare of the proximal CA1 and the molecular layer of distal subiculum. Panels show representative medium- and high-resolution images of sections from the proximal CA1 (top panels) and the distal subiculum (bottom panels) that were double-labeled with antibodies to Tenm3 (green) and Bassoon (red), a presynaptic active zone marker (left panels) or Tenm3 (green) and Homer1 (red), a postsynaptic scaffolding protein (right panels). For additional experiments and quantifications, see Supplementary Fig. 1a–d. c Tenm3 deletion in the MEC causes loss of Tenm3 staining in hippocampal target areas of MEC neurons. Left, representative images of horizontal sections from Tenm3 cKO mice whose MEC was stereotactically infected with AAVs co-expressing tdTomato (red) with either mutant Cre (ΔCre, as a control, top left panel) or Cre (bottom left panel); sections were stained for Tenm3 (green) and DAPI (blue). Right, representative high-resolution views of the proximal CA1 and distal subiculum taken from the experiment shown in the left panels. Note that the Tenm3 deletion in the MEC abolishes nearly all overlap of tdTomato signal derived from the MEC with the local Tenm3 signal in the CA1 region and subiculum. Scale bars: 0.1 mm (a, left and middle panels), 10 µm (a, right panel), 100 µm (b, left panel in each group), 5 µm (b, right panel in each group), 0.5 mm (c, left panel) and 50 µm (c, right panel).