Biocatalytic conversion of α-diketones and α-hydroxy ketones substrates by (R,R)-BDH of B. clausii and comparison to the specific activity toward reduction of acetoin. Substrates (10 mM) were reduced by 1 U ml⁻¹ BDH using formate (30 mM) and formate dehydrogenase (5 U ml⁻¹) to regenerate NADH (0.3 mM) at 30 °C in MES–NaOH buffer (50 mM, pH 6.8; 1 ml, 60 min). Data for acetoin and diacetyl data were taken from Muschallik et al.²⁴.

Substrate	Biocatalytic conversion [%]	Identified products	ee/de [%]
	99		11
	99		99
	90		—
	99		89
	88		99
	99		97
	99		99
	99		99
	99		99
	97		86