Fig. 5.

EMT protein expression in SCC-4 cells and DOK cells. DOK and SCC-4 cells were untreated or treated with 10 μg/ml IgG isotype control, 30 ng/ml rhIL-6, 10 μg/ml IL-6 neutralising antibody, and 40 μg/ml IL-6Ra antibody for 24 h. Lysates were collected and samples were run on 8% SDS-PAGE gel. Sample blots for DOK and SCC-4 and collated densitometry data for β-catenin is presented. β-catenin expression is significantly upregulated in SCC-4 untreated group compared to DOK untreated counterparts, while (Fig. S6C) E-cadherin is not expressed in SCC-4 cells, (Fig. S6D) N-cadherin and (Fig. S6E) vimentin protein expression is absent in DOK cells. Results were plotted using Graphpad prism 8. Results represent three experiments performed (mean ± SEM (n)). Statistical analysis was performed using two-way ANOVA to compare mean values among the groups, *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001