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PAPP-A-mediated IGF-dependent IGFBP-4 proteolysis
72-hr CM from NHLF treated without and with TGF-β was assessed for IGF-dependent IGFBP-4 proteolysis by Western blot with radiolabeled IGFBP-4. (A) Intact and fragmented IGFBP-4 are indicated. Inhibition by mAb-1/41 confirmed specificity of PAPP-A-mediated proteolysis. (B) Measurement of initial reaction velocity.
C, control; rPA, recombinant PAPP-A
