Figure 3.

Effect of acute and chronic treatment of NHLF with TGF-β or IGF-I on Akt phosphorylation

(A) IGF-I (10 nM) and TGF-β (10 ng/ml) were incubated with NHLFs to generate 72-hr CM or directly added to unconditioned SFM. These samples were then incubated with fresh NHLF for 15 minutes and cells harvested for Western blotting of phosphorylated Akt (pAkt) and Total Akt (T-Akt).

(B) Representative Western blot of pAkt and T-Akt after treatment with 72-hr CM without or with (+) inhibitor to PAPP-A (mAb-1/41) or IGF-I (Sm1.2) added 30 minutes prior to NHLF stimulation

(C) Results (% of control) are mean ± SEM of three separate experiments. Dotted line indicates 100%, i.e., no inhibition.

*Significant effect of inhibitor by ANOVA, P < 0.05