Fig. 5. A loss of wild-type C-terminal sequence, not a neomorphic sequence, is responsible for frameshift variant phenotypes.

a NCPR (net charge per residue; 5-aa window) of WT and N323Tfs*36 peptides. Positively (blue) and negatively (red) charged amino acids are indicated. b Sequences in WT, +1 frameshift mutants in eoOPMD, Δ323–341, and +2 frameshift mutant (N323Lfs*31). c Localization of FLAG-tagged hnRNPA2 proteins without (left) or with (right) 0.5 mM NaAsO₂. eIF4G was used as a cytoplasmic and stress granule marker. Representative images from three independent experiments are shown. Scale bar, 10 μm. d Representative images of C2C12 cells one (top) or two (bottom) days after change to differentiation media from three independent experiments. Scale bar, 20 μm. e Quantification of GFP-positive cells negative for both PI and annexin V. Values represent means ± s.e.m. (n = 3 independent experiments). ****P < 0.0001 by two-way ANOVA with Dunnett’s multiple comparisons test.