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. 2022 Apr 28;13:2306. doi: 10.1038/s41467-022-30015-1

Fig. 6. Altered nucleocytoplasmic distribution of hnRNPA2 is responsible for the cytotoxicity associated with its frameshift variants.

Fig. 6

a The amino acid sequences of the relevant domains in WT, N323Tfs*36, P318A, Y319A, ΔPY, ΔPY-NLS and N323Tfs*36+cNLS. b Intracellular localization of FLAG-tagged hnRNPA2 proteins. Representative images from three independent experiments are shown. Scale bar, 10 μm. c Representative scatter plots of GFP-positive C2C12 cells stained with propidium iodide (PI) and annexin V, one (top) or two (bottom) days after change to differentiation media. d Quantification of GFP-positive cells that were negative for both PI and annexin V. Values represent means ± s.e.m. (n = 3 independent experiments). ****P < 0.0001 by two-way ANOVA with Dunnett’s multiple comparisons test.