Figure 4.

Versatile platform for the screening of behavior and neuronal responses to chemicals in the adult C. elegans. (a-c) Behavior phenotyping. (a) Serotonin 5-HT dose-response assay performed using the Adder and generated with 20 animals of each genotype at each concentration. (b) Aversive response to osmotic upshift carried out with the liquid exchanger (n = 15 for 400 mM and 800 mM conditions, n = 10 for the control, error bars are SEM). (c) Food-exposure response using a train of droplets alternating buffer and bacteria in the liquid exchanger (n = 15 for stimulus, n = 10 for control, error bars are SEM). (d-f) Monitoring neuronal activity. (d) Fluorescence microscopy set-up for calcium transient imaging in freely swimming animals. (e) Fluorescence images of the chemosensory neuron ASH in a rested state (top) and activated state upon stimulation with 500 mM NaCl solution (bottom). (f) Average calcium response of ASH upon stimulation with 500 mM NaCl solution (red curve) and control (black curve). Shaded areas indicate SEM (n = 20 per condition).