KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Chemicals, Peptides, and Recombinant proteins | ||
| Sodium chloride (NaCl) | Millipore Sigma | Cat# S7653 |
| Potassium chloride (KCl) | Millipore Sigma | Cat# P9333 |
| Calcium chloride (CaCl2) | Millipore Sigma | Cat# 499609 |
| Magnesium chloride (MgCl2) | Millipore Sigma | Cat# M8266 |
| Lithium chloride (LiCl) | Millipore Sigma | Cat# 203637 |
| Sodium acetate (NaAc) | Millipore Sigma | Cat# 241245 |
| Sodium phosphate monobasic dihydrate (NaP) | Millipore Sigma | Cat# 71505 |
| Choline chloride (ChCl) | Millipore Sigma | Cat# C7017 |
| Sucrose | Millipore Sigma | Cat# S7903 |
| D-Glucose | Millipore Sigma | Cat# NIST917C |
| Coumarin (COU) | Millipore Sigma | Cat# C4261 |
| EcoRI | New England BioLabs | Cat# R0101S |
| XbaI | New England BioLabs | Cat# R0145S |
| NdeI | New England BioLabs | Cat# R0111S |
| SapI | New England BioLabs | Cat# R0569S |
| Gibson assembly | New England BioLabs | Cat# E5510S |
| Q5 | New England BioLabs | Cat# M0543S |
| Q5 site-directed mutagenesis kit | New England BioLabs | Cat# E0554S |
| RLT buffer | Qiagen | Cat # 79216 |
| Apex Taq | Genesee Science | Cat #: 42-138 |
| Episcript | Lucigen | ERT12925K |
| Baseline-ZERO DNase | Lucigen | DB0715K |
| Blocking Solution | Roche | Cat# 11921673001 |
| SlowFade Gold antifade | Thermo Fisher | Cat# S36937 |
| Antibodies | ||
| chicken anti-GFP | Abcam | Cat # ab13970 |
| Rabbit anti-RFP | TakaraBio | Cat #632496 |
| mouse anti-Bruchpilot | DSHB | Cat# nc82 |
| donkey anti-mouse AF647 | Invitrogen | Cat #1900251 |
| donkey anti-rabbit AF568 | Invitrogen | Cat # A-11011 |
| goat anti-chicken AF488 | Abcam | Cat # ab150169 |
| Experimental models: Organisms/strains | ||
| D. melanogaster: Ir56bMB09950 | Bloomington Drosophila Stock Center | Stock# 27818 |
| D. melanogaster: wCS; Ir47a1 | Figure 2 | N/A |
| D. melanogaster: wCS; Ir56b1 | Figure 2 | N/A |
| D. melanogaster: wCS; UAS-Ir56b | Figures 2 and 3 | N/A |
| D. melanogaster: wCS; Ir56b-Gal4 | Dr. John Carlson’s lab | Koh et al., 2014 |
| D. melanogaster: wCS; Ir25a2 | Bloomington Drosophila Stock Center | Stock# 41737 |
| D. melanogaster: Ir76b1 | Bloomington Drosophila Stock Center | Stock# 51309 |
| D. melanogaster: wCS; Gr89a-Gal4 | Dr. John Carlson’s lab | Weiss et al., 2011 |
| D. melanogaster: Gr5a-Gal4 | Bloomington Drosophila Stock Center | Stock# 57592 |
| D. melanogaster: w; Gr5a-LexA; UAS-mCD8GFP LexAop-mtdTomato | Dr. John Carlson’s lab | Koh et al., 2014 |
| D. melanogaster: wCS; Gr64f-Gal4 | Dr. John Carlson’s lab | Dahanukar et al., 2007 |
| D. melanogaster: UAS-DTA | Bloomington Drosophila Stock Center | Stock# 25039 |
| D. melanogaster: wCS; Ir56bGal4 | Figure 5 | N/A |
| D. melanogaster: UAS-DmelIr56b | Figure 6 | N/A |
| D. melanogaster: UAS-DereIr56b | Figure 6 | N/A |
| D. melanogaster: UAS-DvirIr56b | Figure 6 | N/A |
| D. melanogaster: UAS-DsimIr56b | Figure 6 | N/A |
| D. melanogaster: UAS-DsecIr56b | Figure 6 | N/A |
| D. melanogaster: UAS-DsuzIr56b | Figure 6 | N/A |
| D. melanogaster: UAS-DmelIr56bTCC | Figure 6 | N/A |
| D. melanogaster: UAS-DmelIr56bΔ51 | Figure 6 | N/A |
| D. melanogaster: UAS-DmelIr56bΔ462 | Figure 6 | N/A |
| Software and algorithms | ||
| AutoSpike 32 software | Syntech | http://www.ockenfels-syntech.com/ |
| BLASTN | NCBI | https://blast.ncbi.nlm.nih.gov/ |
| Clustal Omega | EMBL-EBI | https://www.ebi.ac.uk/Tools/msa/clustalo/ |
| Mview | EMBL-EBI | https://www.ebi.ac.uk/seqdb/confluence/display/THD/Mview |
| SWISS-MODEL | SIB Swiss Institute | https://swissmodel.expasy.org/ |
| NNSPLICE | Berkeley Drosophila Genome Project | https://www.fruitfly.org/seq_tools/splice.html |
| Oligonucleotides | ||
| See Table S3 | This paper | N/A |
| Recombinant DNA | ||
| pCFD5 | Addgene | Plasmid #73914 |
| pHD-DsRed-attP | Addgene | Plasmid #51019 |
| pUAST-attB-QS | Addgene | Plasmid #24366 |
| Ir56b-gRNA-pCFD5 | This paper | N/A |
| Ir56b-HomologyArms-pHD-DsRed-attP | This paper | N/A |
| Ir56b-HomologyArms-Gal4-pHD-DsRed-attP | This paper | N/A |
| DmelIr56b-gene-pUAST-attB-QS | This paper | N/A |
| DsimIr56b-gene-pUAST-attB-QS | This paper | N/A |
| DsecIr56b-gene-pUAST-attB-QS | This paper | N/A |
| DereIr56b-gene-pUAST-attB-QS | This paper | N/A |
| DsuzIr56b-gene-pUAST-attB-QS | This paper | N/A |
| DvirIr56b-gene-pUAST-attB-QS | This paper | N/A |
| DmelIr56b-gene-TTC-pUAST-attB-QS | This paper | N/A |
| DmelIr56b-gene-Δ51-pUAST-attB-QS | This paper | N/A |
| DmelIr56b-gene-Δ462-pUAST-attB-QS | This paper | N/A |
| DmelIr56b-gene-pUAST-attB-QS | This paper | N/A |