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. Author manuscript; available in PMC: 2023 Apr 29.
Published in final edited form as: Circ Res. 2022 Apr 4;130(9):1345–1361. doi: 10.1161/CIRCRESAHA.121.320005

Figure 8. The ablation of DYRK1a promotes the expression of cell cycle genes after I/R MI.

Figure 8.

(A) Volcano plot of gene expression of αMHC-Cre::Fucci2aR cardiomyocytes after I/R MI or Sham surgeries. Red dots represent genes with a more than a 2-fold increase in expression and an adjusted p-value of a more than 0.05. Blue dots represent genes with less than a 2-fold increase in expression and an adjusted p-value of more than 0.05. Black dots represent genes with an adjusted p-value of a more than 0.05. Vertical dashed lines denote four-fold levels of gene expression. The horizontal dashed line denotes an adjusted P-value of 0.05. (B) Volcano plot of gene expression of αMHC-Cre::Fucci2aR::DYRK1aflox/flox cardiomyocytes after Sham or I/R MI. Red dots represent genes with more than 2-fold increase in expression and an adjusted p-value of more than 0.05. Blue dots represent genes with less than 2-fold increase in expression and an adjusted p-value of more than 0.05. Black dots represent genes with an adjusted p-value more than 0.05. Vertical dashed lines denote four-fold levels of gene expression. The horizontal dashed line denotes an adjusted P-value of 0.05. (C) Venn diagram of genes from panels C and D that had more than 2-fold increase in expression and an adjusted p-value of more than 0.05. Eighty-nine genes were uniquely expressed in αMHC-Cre::Fucci2aR::DYRK1aflox/flox cardiomyocytes after I/R MI. Eighty-six genes were uniquely expressed in αMHC-Cre::Fucci2aR cardiomyocytes after I/R MI. Ninety genes had shared expression in αMHC-Cre::Fucci2aR::DYRK1aflox/flox and αMHC-Cre::Fucci2aR cardiomyocytes. (D) Pathway enrichment or the genes in Panel E based on Reactome database. Genes unique to the αMHC-Cre::Fucci2aR::DYRK1aflox/flox cardiomyocytes after I/R MI were enriched in mitotic cell cycle and cell cycle checkpoints. Genes that were unique to the αMHC-Cre::Fucci2aR cardiomyocytes after I/R MI were enriched in Interleukin-10 and RUNX signaling. Genes in common between αMHC-Cre::Fucci2aR::DYRK1aflox/flox and αMHC-Cre::Fucci2aR cardiomyocytes after I/R MI were enriched in collagen formation and extracellular matrix organization. The tables list the Reactome pathway name, the number of genes in panel E found in the Reactome pathway, the number of total genes in the Reactome pathway, the ratio of genes found to total genes in the pathway, the p-value associated with over-represented genes within the Reactome pathway, and the false discovery rate (FDR).