|
| Antibodies |
|
| Rabbit polyclonal anti Vasa |
R. Lehmann |
N/A |
| Goat polyclonal anti Vasa |
Santa Cruz Biotechnology |
Cat# sc-26877 (dC-13), RRID:AB_793880 Discontinued |
| Mouse monoclonal anti Fasciclin III |
Developmental Studies Hybridoma Bank |
DSHB:7G10; RRID:AB_528238 |
| Rabbit polyclonal anti STAT92E |
E. Bach |
N/A |
| Rabbit polyclonal anti RFP |
Abcam |
ab62341; RRID:AB_945213 |
| Mouse monoclonal anti Islet |
Developmental Studies Hybridoma Bank |
DSHB:40.3A4; RRID:AB_528313 |
| Rat monoclonal anti DE-cadherin |
Developmental Studies Hybridoma Bank |
DSHB:DCAD2 RRID: AB_528120 |
| Guinea pig polyclonal anti Traffic jam |
D. Godt |
N/A |
| Mouse monoclonal anti Gamma Tubulin |
Sigma |
GTU-88, T6557 |
| Chick polyclonal anti GFP |
Aves labs |
Cat#GFP-1020; RRID: AB_2307313 |
| Rabbit polyclonal anti Biniou |
E. Furlong |
N/A |
| Normal Donkey Serum (NDS) |
Jackson Immunoresearch Laboratories |
Cat#: 017-000-121; RRID: AB_2337258 |
| Alexafluor Secondary Antibodies (488, 647) |
Molecular Probes |
N/A |
| Cy3 Affinipure Secondary Antibodies |
Jackson ImmunoResearch |
Cat#: 711-165-152; RRID: AB_2307443 |
|
| Chemicals, peptides, and recombinant proteins |
|
| Para-Formaldehyde (PFA), 16% |
Electron Microscopy Sciences |
Cat#15710 |
| Hoechst |
Sigma |
Cat#33342; CAS Number: 875756-97-1 |
| Propyl-gallate |
Propyl-gallate |
SKU: P3130; CAS Number 121-79-9; PubChem Substance ID 24898394 |
| Normal Donkey Serum (NDS) |
Jackson Immunoresearch Laboratories |
017-000-121; RRID: AB_2337258 |
| Normal Goat Serum (NGS) |
Jackson Immunoresearch Laboratories |
Jackson Immunoresearch Laboratories |
| Ringer’s solution |
Other |
https://doi.org/10.1101/pdb.rec12409
|
|
| Critical commercial assays |
|
| Click-iT EdU |
ThermoFisher |
Cat#: C10419
|
|
| Experimental models: Organisms/strains |
|
| six4-nls-eGFP |
D. Finnegan |
Clark et al., 2006 |
| P-Dsix4-eGFP::Moesin |
R. Lehmann |
Sano et al., 2012
|
| nos-moesin::GFP |
R. Lehmann |
Sano et al., 2005; FBtp0040584 |
| His2Av::mRFP1 |
Bloomington Drosophila Stock Center |
FBtp0056035 |
| Prd-Gal4 |
Bloomington Drosophila Stock Center |
FBst0001947 |
| tupAME-Gal4 |
J.-L. Frendo |
N/A |
| UAS-tdTomato |
Bloomington Drosophila Stock Center |
FBst0036328 |
| trh8 / TM3, twi-Gal4, UAS-GFP |
D. Andrew |
FBal0050667 |
| y1 w*; tup1 P{neoFRT}40A / CyO |
Bloomington Drosophila Stock Center |
FBst0036503; RRID:BDSC_36503 |
| tupex4 |
S. Campuzano |
FBal0216723 |
| TM3, P{w[+mC]=GAL4-twi.G}2.3, P{UAS-2xEGFP}AH2.3, Sb[1] Ser[1] |
Bloomington Drosophila Stock Center |
FBst0006663; RRID:BDSC_6663 |
| w1118 |
Bloomington Drosophila Stock Center |
BL#3605; RRID:BDSC_3605 |
| upd-Gal4 |
E. Matunis |
N/A |
|
biniou[R22}
|
M. Frasch |
Zaffran et al., 2001; FBal0043738 |
|
biniou[I1]
|
M. Frasch |
Zaffran et al., 2001; FBal0043737 |
| UAS-mcd8GFP |
Bloomington Drosophila Stock Center |
FBtp0002652 |
|
jeb[weli] |
A. Holz |
Stute et al., 2004; FBal0159133 |
| Df(2R)BSC699 |
Bloomington Drosophila Stock Center |
FBab0045764 |
| Df(2R)BSC25 |
Bloomington Drosophila Stock Center |
FBab0029944 |
|
slit[2]
|
Bloomington Drosophila Stock Center |
Battye et al., 1999; FBal0015700 |
|
htl[AB42]
|
Bloomington Drosophila Stock Center |
FBal0057264 |
|
hlh54f[delta598]
|
Ismat et al., 2010
|
FBal0248839 |
|
twi-Gal4 |
Bloomington Drosophila Stock Center |
FBtp0000706 |
| UAS-sli.D
|
Bloomington Drosophila Stock Center |
FBti0186586 |
| UAS-ths289.22 |
A. Stathopoulos |
Kadam et al., 2012
|
|
pyr-Gal4 |
Bloomington Drosophila Stock Center |
FBti0212964; Lee et al., 2018. A gene-specific T2A-GAL4 library for Drosophila. eLife 7:e35574 |
| ths-Gal4 |
Bloomington Drosophila Stock Center |
FBti0196141; Wu et al., 2017. Fibroblast growth factor signaling instructs ensheathing glia wrapping of Drosophila olfactory glomeruli. PNAS 114: 7505-7512 |
| UAS-red Stinger |
Bloomington Drosophila Stock Center |
FBti0040830 |
| Htl-mCherry |
A. Stathopoulos |
Irizarry and Stathopoulos, 2015 |
|
robo2[X123]
|
G. Bashaw |
FBal0123720 |
|
robo1[GA285]
|
G. Bashaw |
FBal0032588 |
| slit-LacZ |
Bloomington Drosophila Stock Center |
FBti0006990 |
| Slit::GFP |
Bloomington Drosophila Stock Center |
FBti0181864 |
|
| Recombinant DNA |
|
| GAA GAA TCC CAG CAA AGA CCG TGA GTTG |
Clark et al., 2006 |
D-six4 third intron, forward, EcooRI site |
| GTT GGA TCC ATT GCC ATC CAG TTG |
Clark et al., 2006 |
D-six4 third intron, reverse, BamH1 site |
|
| Software and algorithms |
|
| FIJI (Image J) |
www.fiji.sc
|
N/A |
| Image J |
www.imagej.nih.gov/ij/
|
N/A |
| Metamorph Microscopy Automation and Image Analysis Software |
Leica;https://www.moleculardevices.com/products/cellular-imaging-systems/acquisition-and-analysis-software/metamorph-microscopy
|
v7.8.40; RRID:SCR_002368 |
| Axio-Vision Imaging Software |
Zeiss |
v4.8.1 |
| Adobe Photoshop |
https://www.adobe.com/uk/products/photoshop.html
|
RRID:SCR_014199 |
| Graphpad Prism |
Graphpad Software |
v7.0; RRID:SCR_002798 |
|
| Other |
|
| Matek imaging dish |
Thermofisher |
P35G-1.5-14-C |
| Leica DM16000 B inverted spinning disk confocal |
Leica |
N/A |
| 63x / 1.2 NA water immersion objective |
Leica |
506279 |
| 40x / 1.1 NA water immersion objective |
Leica |
N/A |
| Leica M165FC |
Leica |
N/A |
| Leica M165C |
Leica |
N/A |
| GFP Filter set ET470/40x; ET525/50m |
Leica 10447408 |
N/A |
| mCherry Filter set ET560/40x; ET630/75m |
Leica 10450195 |
N/A |
| DAPIFilter set AT350/50x; ET460/50m |
Leica 10450196 |
N/A |
| Achromat 1.6x objective |
Leica 10450163 |
N/A |
| Video 0.63x objective |
Leica 10447367 |
N/A |
| Needle holder |
Fisher Scientific |
N/A |
| Nytex basket |
|
N/A |
