Figure 7.

Prenatal baclofen administration corrected the defects in dendritic spines density of pyramidal neurons in the mPFC in F2 mice. (A) A composite of a representative scanned image of Golgi-Cox-stained slices of the mPFC and the mouse brain map from The Mouse Brain in Stereotaxic Coordinates; scale bar: 500 and 40 μm. (B) The part inside the yellow rectangle is the neurons and dendrites selected from layer V in the mPFC for analysis. The spines were selected from grade 3 basal dendrites for analysis. The yellow arrow points to a mushroom spine, and the blue arrow points to a stubby spine. Scale bar: 5 μm. (C,D) Summary of spine density on the basal dendrites of mPFC pyramidal neurons. Mature spines = mushroom spines + stubby spines (CTRL: n = 24 dendrites from 3 mice; F2-Con: n = 27 dendrites from 3 mice; F2-Int: n = 26 dendrites from 3 mice). One-way ANOVA followed by the Bonferroni post-hoc test was used to compare the differences among the four groups; *p < 0.05, **p < 0.01, ***p < 0.001. All data for all figures are plotted as the mean ± SEM.