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. 2022 Apr 28;12:6977. doi: 10.1038/s41598-022-10996-1

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Protein–protein interaction (PPI) analysis of N-glycoprotein. The protein interaction network diagram drawn by Cytoscape software (http://www.cytoscape.org/, version 3.2.1) is based on the N-glycoproteins corresponding to the differentially expressed N-glycosylated peptides fragments. The nodes indicated the differentially expressed N-glycoprotein and the connecting lines indicated the interactions between proteins. Down-regulated proteins are shown in blue while upregulated genes are shown in red. Proteins possessing multiple N-glycopeptides which were oppositely regulated are marked in gray. The size of the circle indicates the number of proteins directly interacting with a protein.