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. 2022 Apr 28;12:6915. doi: 10.1038/s41598-022-10826-4

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Images of protein from crude extract of aL3Gs subjected to two-dimensional electrophoresis (2-DE). Using IEF, the proteins are separated on a linear pH range of 3–10 in the first dimension and followed by 12% SDS-PAGE for the second dimension. The gel on the left is stained with Coomassie blue (A). The right side shows the nitrocellulose membrane with proteins electro-transferred from the gel and then probed with pooled sera from patients diagnosed with gnathostomiasis (B). Numbers at the left of each image indicate protein molecular weight (MW) markers.