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. 2022 Apr 28;12:6915. doi: 10.1038/s41598-022-10826-4

Table 5.

List of proteins identified from aL3Gs after 2D-immunoblot analysis to identify proteins of importance related to diagnostic development of gnathostomiasis infection.

Gel No Protein description Score M.W No. of sequence %cov
1 1 Unknown 63 28,771 4 16.2
2 Unknown 42 54,746 1 1.6
2 1 Unknown 70 28,771 4 19.7
2 Cytochrome c oxidase assembly protein cox15-like protein 30 64,100 3 7.5
3 Grip and coiled-coil domain-containing protein 2 30 80,345 2 3.4
4 f-box wd repeat-containing protein 5 29 21,484 1 5
5 Hemicentin-1 25 9709 1 8.5
6 unknown 24 71,901 1 3.1
3 1 unknown 37 28,771 2 7.7
2 unknown 35 9753 1 9.2
3 unknown 26 8063 1 21.9
4 1 unknown 130 28,771 4 16.2
2 Translational activator GCN1 55 293,891 4 2.3
3 Adenosine monophosphate-protein transferase FICD 52 92,486 3 2.9
4 Otopetrin-1 48 67,029 2 2.8
5 1 Otopetrin-1 74 131,621 4 4.8
2 Nucleoside-diphosphatase mig- 55 58,592 2 3.6
3 Anaphase-promoting complex subunit 5 52 119,152 3 2.7
4 Trafficking protein particle complex subunit 8 51 154,261 3 2.8
5 zinc metallopeptidase 2 MEP2 48 74,140 3 5
6 1 Steroidogenic acute regulatory-like protein 1 132 43,117 11 19.7
2 Ankyrin-2 36 74,803 1 2.4
3 Methionine synthase reductase 30 90,007 1 1.1
4 Cytochrome c oxidase assembly protein cox15-like protein 30 63,522 2 3.3
5 Grip and coiled-coil domain-containing protein 2 29 80,285 2 2.1
6 Transcription factor Dp- 23 110,765 2 2.6
7 sn1-specific diacylglycerol lipase beta 19 80,114 2 2.5
7 1 Unknown 78 28,771 2 6.6
2 Adenosine monophosphate-protein transferase FICD homolog 55 92,486 2 1.8
8 1 32 kDa beta-galactoside-binding lectin 892 43,181 17 34.7
2 Unknown 52 13,040 1 7.4
3 Alkylated DNA repair protein alkB homolog 8 48 39,451 2 2.5
4 Unknown 48 25,280 1 3.5
5 Mitogen-activated protein kinase 15 48 117,237 1 0.7
6 Unknown 35 54,746 1 1.6
7 sh2 domain-containing protein 3c 34 86,816 2 2.3
8 Cytochrome c oxidase assembly protein cox15-like protein 29 63,522 1 1.4
9 Protein HID1 29 194,912 3 1.6
10 Ubiquitin carboxyl-terminal hydrolase 31 29 174,633 1 0.5
11 Grip and coiled-coil domain-containing protein 2 29 77,955 1 1.2
12 e3 ubiquitin-protein ligase trim13 27 92,227 1 1.1
13 putative ubiquitin carboxyl-terminal hydrolase 46 23 83,972 1 1.2
14 Sestrin-1 23 45,954 1 2.1
15 Metallophosphoesterase 23 111,504 3 3.2
16 Ras GTPase-activating protein gap-2 23 195,165 1 0.6
17 1-acyl-sn-glycerol-3-phosphate acyltransferase alpha 23 77,675 1 1.3
18 2-oxoglutarate 23 115,162 1 0.9
19 Magnesium transporter 1 23 108,173 1 0.9
20 Homeobox protein cut-like ceh- 23 120,530 1 0.9
21 Putative leucine carboxyl methyltransferase 1 22 69,138 1 1.1
9 1 Fructose-bisphosphate aldolase 1 79 53,610 4 12.6
2 Unknown 64 151,052 4 2.7
3 Spectrin beta chain 63 337,895 5 1.9
4 Proteasome activator complex subunit 4 52 168,147 4 3.5
5 Nucleoside-diphosphatase mig-23 51 58,592 2 3.6
6 Uncharacterized protein F54D1.6 51 181,401 3 2.3
7 Uncharacterized protein DDB_G0271670 50 170,273 4 3.3
10 1 Unknown 33 54,746 1 1.6
2 Protogenin B (Fragment) 27 239,030 2 1.3
3 Probable phosphoserine aminotransferase 16 71,802 1 2.8
4 2-methoxy-6-polyprenyl- -benzoquinol 16 74,095 1 2.6
5 von Willebrand factor and Proteinase inhibitor I15 domain-containing protein 16 197,901 1 0.9
6 Unknown 16 27,113 1 7
7 Decaprenyl-diphosphate synthase subunit 1 16 70,245 1 3
11 1 Fructose-bisphosphate aldolase 1 344 56,589 12 23.4
2 Fructose-bisphosphate aldolase 1 198 53,610 10 23.5
3 Unknown 41 54,746 1 1.6
4 Myosin-2 33 21,125 1 7.1
5 Transcriptional regulator ATRX 21 100,673 1 1.2
12 1 Neuroserpin 68 274,509 2 0.6
3 DNA polymerase kappa 35 53,850 1 1.8
2 Unknown 35 9753 1 9.2
13 1 DNA polymerase kappa 28 53,850 1 1.8
14 - - - - - -
15 1 Putative helicase mot1 74 265,846 5 2.5
2 Protein split ends 60 310,778 5 2.2
3 Lon protease homolog, mitochondrial 52 277,509 3 1.1
4 NAD(P) transhydrogenase, mitochondrial 51 128,218 4 5.1
16 1 Unknown 39 54,746 1 1.6
17 1 Unknown 40 30,189 1 3.7
2 Polyphosphoinositide phosphatase 34 134,822 1 0.7
3 Unknown 25 33,690 1 4.3
4 UPF0378 protein 25 295,143 2 1
5 Heat shock factor binding protein 1 25 44,624 1 4.9
6 Unknown 22 7327 1 9.1
7 Nuclear pore complex protein nup160 22 182,675 1 0.7
8 Unknown 17 51,481 1 2.8
9 Unknown 17 15,910 1 9.5
10 Transgelin-2 17 38,743 1 4.2
11 Unknown 17 27,190 1 4.4
12 Multiple C2 and transmembrane domain-containing protein 1 17 117,365 2 2.5
13 Tyrosine-protein phosphatase non-receptor type 13 17 212,990 1 0.7
14 Reverse transcriptase 17 69,975 1 2.2
15 Unknown 17 36,926 1 4.2
16 Unknown 17 12,421 1 12.1
17 Unknown 17 8180 1 18.7
18 1 Major antigen 74 250,951 3 1.9
2 Hypothetical protein LOAG_11590 20 11,291 1 21.3
19 1 Calponin homolog OV9M 41 72,439 4 8
2 Unknown 36 8848 1 19.8
3 Unknown 34 54,746 1 1.6
4 Major antigen 33 250,951 1 0.5
5 Inositol polyphosphate multikinase 29 55,418 1 1.4
6 Unknown 29 7351 1 10
7 Unknown 29 12,755 1 6.3
8 Acetyl-CoA acetyltransferase, mitochondrial 27 72,535 1 1.8
20 1 Collagenase 3 144 31,095 6 21.5
2 Small heat shock protein ov25-1 54 31,353 1 3.1
3 unknown 42 30,189 2 11.5
21 1 Collagenase 3 323 31,095 7 28
2 Hypothetical protein LOAG_06805 40 25,230 1 4.2
3 Unknown 37 13,565 1 17.4
4 Unknown 35 54,746 1 1.6
5 sh2 domain-containing protein 3c 34 86,816 1 1
22 1 Collagenase 3 64 31,095 4 13.8
2 e3 ubiquitin-protein ligase trim13 29 92,227 2 3.1
3 Unknown 22 42,073 1 3.4
4 Isocitrate isopropylmalate dehydrogenase domain-containing protein 17 67,366 1 2.3
5 PREDICTED: 5-oxoprolinase 17 102,576 2 3.7
6 putative f-box lrr-repeat protein 17 45,640 1 3.1
7 N-alpha-acetyltransferase 60 17 19,499 1 7.9
8 Unknown 17 17,177 1 7.8
9 Hypothetical protein WUBG_00429 17 55,682 1 2.4
10 Hypothetical protein DICPUDRAFT_160192 17 7537 1 18.3
11 Unknown 17 13,878 1 11.1
12 Cathepsin B 17 73,569 1 2.1
23 1 Unknown 36 22,949 1 5.6
2 WD-repeat protein 22 33 113,846 1 0.8
3 sh2 domain-containing protein 3c 29 86,816 2 2.3
4 Unknown 29 54,746 1 1.6
5 Glutamate-gated chloride channel subunit beta 29 67,212 1 1.3
6 Unconventional myosin-IXb 23 231,645 1 0.7
7 Na-dependent Cl/HCO3 exchanger 17 134,665 1 1.2
24 1 Intermediate filament protein B 95 83,781 4 7
2 Unknown 32 32,006 1 5.6
3 Mucin-5AC 19 141,479 1 0.6
4 Hypothetical protein Y032_0138g2053 19 114,441 1 0.7
5 Kinesin-like protein KIF3B 19 8000 1 10.3
6 Cytochrome b5 reductase 4 19 36,260 1 2.6
7 Histone-lysine N-methyltransferase SETMAR 19 22,618 1 3.8