Table 6.

The protein properties of G. spinigerum matrix metalloproteinase-like protein and cathepsin B.

Properties	Software	Matrix metalloproteinase-like protein	Cathepsin B
Molecular weight	Expasy Compute pI/Mw tool	27,661.1 Da	52,709.06 Da
Isolectric point	Expasy Compute pI/Mw tool	7.09	8.54
Signal peptide	SignalP 5.1	Cleavage site between pos. 23 and 24	Cleavage site between pos. 31 and 32
Non-classical secretory	SecretomeP-2.0 Server	Containing signal peptide	Containing signal peptide
Transmembrane protein	TMHMM—2.0	Inside ( pos 1–6), Tmhelix (pos 7–26), outside (27–245)	Outside (1–462)
N-glycosylation	NetNGlyc—1.0	position 41, 59	Position 156, 243
O-glycosylation	NetOGlyc—4.0	position 220, 226, 230, 234, 236	Position 175, 301, 303, 304, 315, 456
% similarity	Clustal omega	28.74% with VDM58434.1 of Angiostrongylus spp.	62.21% with VDM57237.1 of Angiostrongylusspp.
		30.29% with XP_024501568.1 of Strongyloides spp.	64.16% with XP_024507370.1 of Strongyloides spp.
		30.40% with VZI42166.1 of Sparganum spp.	34.38% with VZI02086.1 of Sparganum spp.
		31.9% with NP_002414.1 of H. sapiens	46.91% with NP_071447.1 of H. sapiens
Gene ontology	UniProt	Hydrolase, Protease and Metal-binding	Cysteine-type peptidase activity
Molecular weight	Expasy Compute pI/Mw tool	27,661.1 Da	52,709.06 Da
Isolectric point	Expasy Compute pI/Mw tool	7.09	8.54
Signal peptide	SignalP 5.1	Cleavage site between pos. 23 and 24	Cleavage site between pos. 31 and 32
Non-classical secretory	SecretomeP-2.0 Server	Containing signal peptide	Containing signal peptide
Transmembrane protein	TMHMM—2.0	Inside ( pos 1–6), Tmhelix (pos 7–26), outside (27–245)	Outside (1–462)
N-glycosylation	NetNGlyc—1.0	Position 41, 59	Position 156, 243
O-glycosylation	NetOGlyc—4.0	Position 220, 226, 230, 234, 236	Position 175, 301, 303, 304, 315, 456