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. 2021 Oct 13;42(4):613–629. doi: 10.1177/0271678X211048981

Figure 1.

Figure 1.

Overexpression of Pdzrn3 (iECOE) specifically in endothelial cells. (a) Cre mouse line for overexpression of Pdzrn3 in EC: (A) Breeding scheme for the generation of a Pdgf-iCre/ROSA/LNL:tTA/tet-Pdzrn3; LacZ (Pdzrn3 iECOE) triple transgenic mouse (b) Confocal immunofluorescent images of β galactosidase expression (green) in podocalyxin labeled endothelial cells (red) in hippocampus, cortex and cerebellum sections of transgenic Pdzrn3 iECOE mice, revealing endothelial specific cre-mediated transgene activation. Scale bar = 20 µm. (c) Quantification of the number of podocalyxin positive ECs showing Pdgfb-icre recombination efficacy. (d) Vessel fragments were isolated from harvested mouse brain and immunolabeled with CD31 (red), Podocalyxin (light blue) and Dapi(blue). Scale bar = 10 µm. (e) Vessel fragments were subjected to qRT-PCR. Representative immunoblot images (f) and quantification (g) of TJ proteins Occludin, Claudin5, AJ protein Ve cadherin and of β catenin in control and Pdzrn3 iECOE brain subcellular microvessel fractions: cytosol (C), Membrane (M), nucleus (N), cytoskeleton (K). n = 5. Data are represented as individual values plus means ± SD. Mann-Whitney test was performed. *p < 0.05.