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. 2022 Apr 28;12:6919. doi: 10.1038/s41598-022-10905-6

Figure 5.

Figure 5

Pressure preconditioning increased tumor size in A549 xenograft mice, which was blocked by Pembrolizumab. (A) Pressure preconditioning increased tumor size in A549 xenograft mice. N indicates the number of mice and P indicates the P values by unpaired t test. A549 xenograft tumors were established subcutaneously in athymic nude mice and tumor size was examined every 3 days. (B) PD-L1 antibody Pembrolizumab did not affect tumor size in the absence of αROR1-CAR T cells. Pembrolizumab (5 mg/kg) was injected intravenously on day-21. (C) Pressure preconditioning increased tumor size in the presence of αROR1-CAR T cells. αROR1-CAR T cells were injected on day-21 and day-27. (D) Pembrolizumab enhanced the efficacy of αROR1-CAR T cells. Pembrolizumab (5 mg/kg) was injected intravenously on day-21 and day-27. Green arrows indicate adding Pembrolizumab and yellow CAR-T cells. (E) Tumors were surgically removed after sacrificing the mice on day-33 and imaged. (F) Tumors were weighed and statistically analyzed by unpaired t test. ns not significant. (G) CAR T cells and Pembrolizumab treatment did not affect the bodyweight of xenograft mice. (H) Plasma TNF-α levels were increased by combined treatment of CAR-T cells and Pembrolizumab.