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. 2022 Apr 15;13:841171. doi: 10.3389/fmicb.2022.841171

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Copyright © 2022 Wang, Zhang, Du, Zhou and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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BsMinC and BsMinD coassembled into a long and large bundle in the ratio of 1:1. (A) Negative stain EM showed that 8 μM BsMinD and 4 μM BsMinC coassembled into large bundles in the presence of 2 mM ATP. Bar represents 200 nm. (B) SDS-PAGE analysis of cosedimentation in different ratios of BsMinC and BsMinD showed that the ratio of BsMinC and BsMinD in the pellet is always approximately 1:1. The initial concentration of the mixture, the estimated MinC and MinD concentrations in the supernatant and pellet are presented at the bottom of the panel. S, supernatant; P, pellet.