Figure 3.

Endogenous enzymes are not responsible for elevated kynurenine in TC mice

(A) Depiction of the endogenous enzymes and metabolites (blue) in the kynurenine and serotonin pathways.

(B) Ido1 gene expression in B6 and TC CD4⁺ T cells.

(C) Representative flow cytometry histogram overlays of IDO1 mean fluorescence intensity (MFI) in immune cell subsets from B6 and TC mice.

(D) Quantitation of (C), N = 6.

(E) Western blot analysis of B6 and TC CD4⁺ T cells (N = 3) for IDO1 and β-actin. Epididymis (Ep) from B6 and B6.Ido1^−/− mice were used as positive and negative controls, respectively.

(F) Expression of KP genes in B6 and TC livers relative to Ppia, N = 8–13.

(G) Concentration of KMO protein in B6 and TC liver, N = 8. B, D–G: C–J: Graphs show individual mice with mean ± SEM t tests, ∗∗∗p < 0.001.