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. 2022 Apr 29;12(4):e752. doi: 10.1002/ctm2.752

FIGURE 4.

FIGURE 4

LINC01606 affected the SCD1 level by interacting with miR‐423‐5p. (A) The location of LINC01606 in colon cancer cells by FISH assays. Scale bars 2.5 μm. (B) The binding sites of miR‐423‐5p on LINC01606 and SCD1 3′UTR. (C) Protein and mRNA expression level of SCD1 in SW480 and HT29 cells after transfection with sh‐LINC01606‐expressing vector or LINC01606‐expressing vector (n = 3). (D) mRNA expression level of LINC01606 and protein and mRNA expression level of SCD1 in SW480 and HT29 cells after transfection with miR‐423‐5p mimics or inhibitors (n = 3). (E) Luciferase reporting gene assay was performed to analyse whether miR‐423‐5p could bind to LINC01606 and SCD1 3′UTR (n = 3). (F and G) Interaction of miR‐423‐5p with lncRNA LINC01606 and SCD1 was investigated through the RIP and pull‐down assays in SW480 (n = 3). Data are shown as the mean ± SD. ns, no significant (p > .05), *p < .05, **p < .01 and ***p < .001 compared with control