FIGURE 6.

LINC01606 suppresses colon cancer cell ferroptosis. (A) Levels of intracellular total iron and ferrous iron (Fe²⁺) were analysed in SW480 and HT29 cells subjected to the stable knockdown of LINC001606 or stably overexpressing LINC001606 after treatment of DMSO, Erastin (10 μM) and RSL3 (2 μM) for 48 h (n = 3). (B) Representative images of intracellular mitochondrial superoxide level in SW480 and HT29 cells subjected to the stable knockdown of LINC001606 or stably overexpressing LINC001606 after treatment of DMSO, Erastin (10 μM) and RSL3 (2 μM) for 48 h. Scale bars 2.5 μm (n = 3). (C) Levels of mitochondrial membrane potential were analysed in SW480 and HT29 cells subjected to the stable knockdown of LINC001606 or stably overexpressing LINC001606 after treatment of DMSO, Erastin (10 μM) and RSL3 (2 μM) for 48 h (n = 3). (D) Flow cytometry analysis the relative levels of intracellular lipid ROS in SW480 and HT29 cells subjected to the stable knockdown of LINC001606 or stably overexpressing LINC001606 after treatment of DMSO, Erastin (10 μM) and RSL3 (2 μM) for 48 h (n = 3). Data are shown as the mean ± SD. *p < .05, **p < .01 and ***p < .001 compared with control