FIGURE 11.

Identification of transcription factor TFE3 binding to the LINC01606 promoter. (A) Transcription related‐nucleoprotein binding to the LINC01606 promoter was detected by DNA pull‐down and LC–MS in SW480 cells. (B) Gene Ontology enrichment analysis of differentially expressed of nucleoprotein binding to LINC01606 promoter region. (C) Protein and mRNA expression level of TFE3 in TCGA colon rectal cancer tissues and normal tissues. (D) TFE3 expression levels were assessed in human colon cancer tissues compared with the paired adjacent normal tissues (p < .001, n = 83). Association between TFE3 levels and LINC01606 levels in 83 colon cancer patients. (E) Relative expression of LINC01606 in SW480 cells transfected with TFE3 or control plasmid (n = 3). Expression levels were normalised to GAPDH levels. (F) JASPAR was used to predict sites of putative TFE3 binding motif in the LINC01606 promoter region. Luciferase reporting gene assay was performed to analyse whether TFE3 could bind to LINC01606 promoter region (n = 3). (G) ENCODE database predicted the TFE3 Chip‐seq signal values on LINC01606 promoter region in A562 and HepG2 cells. Data are shown as the mean ± SD. *p < .05, **p < .01 and ***p < .001 compared with control. TSS, transcription start site; TF, transcription factor