TABLE 2.
Effect of CLR on the generation times of MAC strains
| Strain | Phenotypea | Generation time (h [mean ± SE]) with the following CLR concn (μg/ml):
|
||
|---|---|---|---|---|
| 0 | 4 | 64 | ||
| 101R | CLRr | 15.0 ± 2.2 | 12.4 ± 0.4 | 14.5 ± 2.4 |
| JJL004.2 | CLRr | 15.7 ± 0.5 | 15.2 ± 0.3 | 15.8 ± 0.4 |
| 511 | CLRr | 13.3 ± 0.3 | 13.1 ± 0.4 | 14.7 ± 0.5 |
| 512 | CLRr | 11.4 ± 0.9 | 11.3 ± 0.3 | 12.0 ± 1.2 |
| 504ER-1 | CLRr | 12.2 ± 0.7 | 13.4 ± 1.2 | 14.7 ± 1.1 |
| 504ER-2 | CLRr | 11.2 ± 0.3 | 11.0 ± 0.1 | 11.3 ± 0.3 |
| 511/504b | CLRr/CLRs | 13.5 ± 0.1 | 13.2 ± 0.6 | 13.3 ± 0.3 |
| 101 | CLRs | 8.3 ± 0.1 | —c | — |
| 504 | CLRs | 9.8 ± 0.5 | — | — |
a
For CLRr strains the MIC is ≥64 μg/ml; for CLRs strains the MIC is ≤4 μg/ml. All strains carry a 23S rRNA gene mutation, assessed either by mismatch assay (strains 504ER-1 and 504ER-2) or by DNA sequencing (14).
b
Mixture of strain 511 and strain 504 in an initial CFU ratio of 1:106. Generation times are for strain 511.
c
—, CLR concentrations of ≥4 μg/ml were bactericidal for MAC strains 101 and 504.