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. 2022 Mar 30;298(5):101885. doi: 10.1016/j.jbc.2022.101885

Figure 7.

Figure 7

Overexpression of hCAR represses EPO downstream signaling in HepG2 and Hep3B cells.A, schematic illustration of the EPO–EPOR signaling pathway. B and C, HepG2-hCAR and Hep3B-hCAR cells were treated with vehicle control or Dox (1 μg/ml) for 72 h before Western blotting analysis of total and phosphorylation of STAT3 (p-STAT3-Y705), AKT (p-AKT-S473), and ERK1/2 (p-ERK1/2-T183/Y185). D, relative blot densitometry was quantified using ImageJ from three separately prepared cell experiments and normalized to the density of the loading control. Data are expressed as mean ± SD (n = 3). ∗p < 0.05 and ∗∗p < 0.01. Dox, doxycycline; EPO, erythropoietin; EPOR, EPO receptor; ERK1/2, extracellular signal–regulated kinase 1/2; hCAR, human constitutive androstane receptor; STAT3, signal transducer and activator of transcription 3.