Summary of the fluorescence recovery ratio (F/F0) of Cu-dpa-QZ1 and Cu-dpa-QZ2 in the presence of FPB-γ-CyD with various phosphoric acid derivatives against the initial state at fluorescence maximum wavelengtha.
| Phosphoric acid derivatives | Fluorescence recovery ratio (F/F0)b | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Cu-dpa-QZ1 | Cu-dpa-QZ2 | |||||||||
| None | β-CyD | γ-CyD | FPB-β-CyD | FPB-γ-CyD | None | β-CyD | γ-CyD | FPB-β-CyD | FPB-γ-CyD | |
| Pi | 2.8 | 2.5 | 1.9 | 3.1 | 2.4 | 6.2 | 2.5 | 2.1 | 4.3 | 2.9 |
| PPi | 4.5 | 3.9 | 2.6 | 8.7 | 5.5 | 55 | 14 | 14 | 65 | 133 |
| Tri | 4.6 | 3.9 | 2.7 | 8.6 | 5.3 | 55 | 14 | 14 | 64 | 135 |
| AMP | 1 | 1.2 | <1 | 1.5 | 1.3 | <1 | <1 | <1 | <1 | <1 |
| ADP | 4.0 | 3.6 | 2.5 | 6.6 | 4.5 | 19 | 8.3 | 6.2 | 16 | 18 |
| ATP | 4.5 | 3.7 | 2.6 | 8.1 | 5.2 | 44 | 12 | 12 | 47 | 69 |
a
Fluorescence measurements for the selectivity of phosphoric acid derivatives were performed in 1% DMSO – 99% water (v/v), pH 7.4 adjusted by HEPES/NaOH buffer, at 25 °C (λdpa-QZ1ex = 355 nm, λdpa-QZ2ex = 345 nm). [probe] = 10 μM, [Cu(NO3)2] = 20 μM, [phosphoric acids] = 1.0 mM, [NaNO3] = 0.10 M, [HEPES buffer] = 5.0 mM.
b
Unless otherwise noted, F/F0 indicates the relative fluorescence intensity (F) at 431 nm of Cu-dpa-QZ1 and 486 nm of Cu-dpa-QZ2 in the presence of 1.0 mM of the phosphoric acid derivatives against that of the initial state (F0).