Table 1.
Primer | Sequence | Amplified Product | Amplification (35 Cycles) | References | ||
---|---|---|---|---|---|---|
Denaturation | Annealing | Extension | ||||
gyrB | F: GAA GTC ATC ATG ACC GTT CTG CAY GCN GGN GGN AAR TTY GA R: AGC AGG ATA CGG ATG TGC GAG CCR TCN ACR TCN GCR TCN GTC AT |
1350 bp | 94°C 1 min. |
66°C 1 min. |
72°C 1 min. |
[32] |
nhe | F:AAG CIG CTC TTC GIA TTC R: ITI GTT GAA ATA AGC TGT GG |
766 bp | 94°C 30 sec. |
49°C 40 sec. |
72°C 45 sec. |
[41] |
cytK | F: ACA GAT ATC GGI CAA AAT GC R: CAA GTI ACT TGA CCI GTT GC |
421 bp | 94°C 30 sec. |
49°C 40 sec. |
72°C 45 sec. |
|
ces | F: GGTGACACATTATCATATAAGGTG R: GTAAGCGAACCTGTCTGTAACAACA |
1271 bp | ||||
pc-plc | F: GAGTTAGAGAACGGTATTTATGCTGC R: CTACTGCCGCTCCATGAATCC |
411 bp | 94°C 1 min. |
55°C 1 min. |
72°C 1 min. |
[42] |
hbl | F: TGCACAAGAAACGACCGCTCA R: ATAATTTGCGCCCATTGTATTCCAT |
987 bp | 94°C 30 sec. |
54°C 40 sec. |
72°C 45 sec. |
[43] |
bla1 | F: CATTGCAAGTTGAAGCGAAA R:TGTCCCGTAACTTCCAGCTC |
680 bp | 94°C 30 sec. |
50°C 40 sec. |
72°C 45 sec. |
[44] |
bla2 | F: TTGTCGATTCTTCTTGGGATG R: CCCCTACTTCTCCATGACCA |
483 bp | ||||
tetA | F: GGCGGTCTTCTTCATCATGC R: CGGCAGGCAGAGCAAGTAGA |
502 bp | 95°C 1 min. |
58°C 1 min. |
72°C 1 min. |
[45] |
ermA | F:TCTAAAAAGCATGTAAAAGAA R:CTTCGATAGTTTATTAATATTAGT | 645 bp | 94°C 30 sec. |
52°C 40 sec. |
72°C 45 sec. |
[46] |