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. 2022 Apr 24;15:2167–2185. doi: 10.2147/IDR.S365254

Table 1.

The Oligonucleotides Sequences Used in the PCR Assay

Primer Sequence Amplified Product Amplification (35 Cycles) References
Denaturation Annealing Extension
gyrB F: GAA GTC ATC ATG ACC GTT CTG CAY GCN GGN GGN AAR TTY GA
R: AGC AGG ATA CGG ATG TGC GAG CCR TCN ACR TCN GCR TCN GTC AT
1350 bp 94°C
1 min.
66°C
1 min.
72°C
1 min.
[32]
nhe F:AAG CIG CTC TTC GIA TTC
R: ITI GTT GAA ATA AGC TGT GG
766 bp 94°C
30 sec.
49°C
40 sec.
72°C
45 sec.
[41]
cytK F: ACA GAT ATC GGI CAA AAT GC
R: CAA GTI ACT TGA CCI GTT GC
421 bp 94°C
30 sec.
49°C
40 sec.
72°C
45 sec.
ces F: GGTGACACATTATCATATAAGGTG
R: GTAAGCGAACCTGTCTGTAACAACA
1271 bp
pc-plc F: GAGTTAGAGAACGGTATTTATGCTGC
R: CTACTGCCGCTCCATGAATCC
411 bp 94°C
1 min.
55°C
1 min.
72°C
1 min.
[42]
hbl F: TGCACAAGAAACGACCGCTCA
R: ATAATTTGCGCCCATTGTATTCCAT
987 bp 94°C
30 sec.
54°C
40 sec.
72°C
45 sec.
[43]
bla1 F: CATTGCAAGTTGAAGCGAAA
R:TGTCCCGTAACTTCCAGCTC
680 bp 94°C
30 sec.
50°C
40 sec.
72°C
45 sec.
[44]
bla2 F: TTGTCGATTCTTCTTGGGATG
R: CCCCTACTTCTCCATGACCA
483 bp
tetA F: GGCGGTCTTCTTCATCATGC
R: CGGCAGGCAGAGCAAGTAGA
502 bp 95°C
1 min.
58°C
1 min.
72°C
1 min.
[45]
ermA F:TCTAAAAAGCATGTAAAAGAA R:CTTCGATAGTTTATTAATATTAGT 645 bp 94°C
30 sec.
52°C
40 sec.
72°C
45 sec.
[46]