Figure 11.

High-resolution native MS of ribosomal particles. (a) (left) Structures of the E. coli 70S ribosome consisting of the 50S (proteins shown in blue and rRNA in green) and 30S (proteins shown in red and rRNA in orange) particles. (right) High-resolution native mass spectra of the 70S, 30S, and 50S ribosomes. (b) Distinct particles of the 30S ribosome, with annotations provided at the top. Masses are shown as mean ± SD. (c) Distinct particles of the 50S ribosome, with annotations provided at the top. In most of the particles, the pentameric stalk complex is absent. Reproduced with permission from ref (66). Copyright 2017 Nature Publishing Group.