Figure 14.

Analysis of adeno-associated virus (AAV) by high-resolution native MS: (A) AAV (structure from PDB entry 3NG9) is composed of 60 copies of a combination of VP1, VP2, and VP3, encoded within the same cap open-reading frame, sharing a common C-terminal sequence. AAV capsid assembly follows a stochastic model, where VP proteins are taken randomly from the pool of expressed VP proteins. The probability for each stoichiometry is determined by the ratio of the expressed VP proteins. (B) Simulated and experimental high-resolution native mass spectra of AAV at mass resolutions corresponding to transient times of 32 and 128 ms. Each plot shows (top to bottom) the simulated mass spectra before and after baseline correction and their experimental counterparts. On the right are depicted zoomed-in parts of these spectra. Adapted from ref (272). CC BY 4.0.