Figure 15.

High-resolution native tandem mass spectra of the designed charge-reduced AaLS-neg particles. (top) The charge-reduced intact Cp180 shows a well-resolved charge-state envelope centered at m/z ≈ 30 000 Th. (middle, bottom) The charge-state envelope was subjected to increasing HCD collision energies of 250 and 300 V. At maximal HCD the mass spectrum revealed the consecutive elimination of monomers from the Cp180-mer with fragment ions up to m/z = 70 000 Th. The obtained mass resolution enabled baseline resolution of these different dissociation products. At the highest m/z, the mass resolution was still greater than 500. The figure is adapted from ref (30). Copyright 2017 Royal Society of Chemistry. The inset structure in the top panel is from ref (282). CC BY 4.0.