Figure 25.

From early tandem MS experiments to IR spectroscopy and ion mobility experiments investigating fucose migration and internal residue loss. (A) Schematic example of fucose migration and internal residue loss for a trisaccharide (upper panel) in the symbol nomenclature for glycans (SNFG) (lower panel, left) and chemical representation of β-l-fucose, a 6-deoxy-l-galactose (lower panel, right). (B) Tandem MS experiments showing unexpected m/z-fragments from internal residue loss. Figure adapted with permission from ref (66). Copyright 2002 American Chemical Society. (C) IR spectroscopy experiments showing fucose migration in the trisaccharides Le^x and BG-H2 as in-source fragmentation products of a tetrasaccharide (upper panel) and as intact parent ions (middle and lower panel). Figure reprinted with permission from ref (205). Copyright 2018 Wiley-VCH. (D) IMS experiments distinguishing two fragments with identical m/z ratio but at least one with a rearranged fucose monosaccharide from their ATD. Figure reprinted with permission from ref (425). Copyright 2019 Wiley-VCH.