Fig. 3.

Leukocyte–endothelial interaction in the brain microcirculation. Rolling A and B adherent rhodamine 6G-labeled leukocytes evaluated by intravital fluorescence microscopy on the cerebral postcapillary venules, monocyte chemoattractant protein-1 (MCP-1) by multiplex immunoassay C and representative images of the brain microcirculation in the cerebral postcapillary venules, arrows indicate leukocytes D of wild-type (WT) and TLR4-mut mice fed a normolipid diet (white bars) or a high-fat diet (black bars) for 24 weeks. Magnification 200×, scale bar 50 µm. Values represent the mean ± SEM. n = 8 ***p < 0.001 vs. WT-normal diet, ^###p < 0.001, versus WT high-fat diet