Fig. 2.

Dexmedetomidine inhibits TLR4/ NF-κB signaling via miR-17-5p in PC12 cells. A miR-17-5p and TLR4 expression in treatment and control groups determined by qRT-PCR. B Relative expression of TLR4, p-IκBα, IκBα, p-p65, and p65 in each group detected by western analysis. Left, protein band; right, relative protein expression level. C Putative binding sites for miR-17-5p in the TLR4 3′-UTR. D Luciferase activities of TLR4 3′-UTR-WT and TLR4 3′-UTR-MUT constructs in HEK293 cells following treatment with NC or miR-17-5p mimic determined using the Dual-Luciferase Reporter Assay system. Data represent the mean ± SD of three independent experiments. *P < 0.05 vs control; #P < 0.05 vs OGD/R + vehicle