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. 2022 Apr 29;22:171. doi: 10.1186/s12890-022-01962-8

Table 1.

Primer sequences used for qRT-PCR analysis of miR-20a-5p, miR-17-5p, miR-93-5p, miR-665 and miR-3202 expression

miRNA Primer sequence (5'–3')
hsa-miR-20a-5p-RT GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCTACCT
JH-hsa-miR-20a-5p-F GCGCGCTAAAGTGCTTATAGTGC
hsa-miR-17-5p-RT GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCTACCT
JH-hsa-miR-17-5p-F GCCAAAGTGCTTACAGTGC
hsa-miR-93-5p-RT GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCTACCT
JH-hsa-miR-93-5p-F GCCAAAGTGCTGTTCGTGC
hsa-miR-665-RT GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAGGGGC
JH-hsa-miR-665-F GCGCACCAGGAGGCTGAG
hsa-miR-3202-RT GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACATTAAA
JH-hsa-miR-3202-F GCTGGAAGGGAGAAGAGC
U6-hF CTCGCTTCGGCAGCACA
U6-hR AACGCTTCACGAATTTGCGT
Universal downstream primer GTGCAGGGTCCGAGGT