Fig. 3.

miR-486-3p directly target Snail. A The mRNA of Snail is detected by qRT-PCR in both cells after 6 Gy irradiation at 0 h, 3 h, 6 h, 48 h. B Detection of protein changes of Snail at 0 h,3 h,6 h,48 h after irradiation in both cells. C Mice (GSE2250) and D human (GSE40839) lung fibrosis models (https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi) show the change of Snail proteins. E The change of miR-486-3p and Snail mRNA levels were checked by qRT-PCR after transfected miR-486-3p inhibitor and mimic. F The protein change of Snail after transfected miR-486-3p inhibitor and mimic. G Putative binding site of miR-486-3p within the 3′UTR region of Snail and mutant sequences. F Results of dual luciferase reporter activity assays in HEK-293T cells. *p < 0.05