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Kinetics of nitrite reduction. (A) YtfE-mediated oxidation of methyl viologen in response to increasing concentrations of nitrite. Note that data for 750 μM were omitted for clarity. (B) Initial rate analyses of data in panel (A). Fitting to a Michaelis–Menten equation (black line) gave a Km of ∼250 μM for nitrite. See Table 1 for full kinetic parameters. Error bars indicate ± standard deviation.
