| strain |
|
|
| RK4353 |
parent strain: lacU169
araD139 rpsL gyrA non
|
(87) |
| JCB5211 |
RK4353 ΔytfE::cat |
(24) |
| JCB5270 |
RK4353 ΔnarGHJI, narZ, hcp, norVW, nrfAB, nirBD, and hmp
|
(27) |
| JCB5280 |
ΔytfE::cat derivative of
JCB5270 |
(27) |
| plasmid |
|
|
| pNF383 |
hcp regulatory
region ligated into pRW50 to give a Phcp::lacZ transcriptional fusion; TetR
|
(6, 16) |
| pBB2016 |
a low copy pACYC184 derivative
expressing ytfE under the control of its own promoter;
TetR and CmR
|
(18, 88) |
| pGS2618 |
a pET24a derivative for
overexpression of His-tagged ytfE; KanR
|
(27) |
| pGS2618CA |
a derivative of pGS2618
for overexpression of the Cya30Ala/Cys31Ala YtfE variant; KanR
|
this work, Genscript |
