Fig. 7. Antimicrobial efficacy of hit compounds (A) anti-urease activity of test compounds was determined after 48 hours of pre-treatment with inhibitor compounds at 100 μg ml⁻¹ concentration in presence of urea broth was tested. Experiment was performed in triplicates and results are expressed as mean of two independent experiments. (B) Bacterial growth in presence of acid stress was monitored by measuring suspension absorbance at 600 nm after 24 hours of incubation in presence of test compounds at (100 μg ml⁻¹). Bacterial growth was monitored at 5 and 3.5 pH. Statistical analysis was performed using unpaired t-test. Results are expressed as mean of two independent experiments. * = p value of < 0.05, ** = p value < 0.005, *** = p value < 0.0005. (C) Bacterial viability was determined using MTT assay after 24 hours after addition of test compounds (100 μg ml⁻¹). Viable cells were qualitatively assessed by development of red coloration after addition of 2,3,5-triphenyltetrazolium chloride (TTC). Deep red color was assigned a score of (3) followed by red indicating less viable cells (2), light pink coloration indicating few alive cells (1) and no viability with color development similar to blank (0). Viability was monitored at 5 and 3.5 pH.