Figure 5.

GECPAR in trans transcriptional regulatory function. (A) Pipeline of CHART experiment and analysis. (B) Panther gene ontology classification of 325 GECPAR target genes identified both in OCI-LY1 and U2932 by CHART. (C) Preranked gene set enrichment analysis (GSEA) of RNA sequencing (RNA-Seq) data after GECPAR knockdown (KD) in U2932. (D) Preranked GSEA of RNA-Seq data after GECPAR overexpression in SUDHL2. (E) Top: Venn diagram crossing genes with GECPAR binding detected by CHART sequencing and significant expression modulation after GECPAR KD in U2932. Direct downregulated (left) and upregulated (right) GECPAR targets are listed. Bottom, preranked GSEA of direct GECPAR positively regulated targets, in germinal center B cell-like diffuse large B-cell lymphoma patients dichotomized for GECPAR expression