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. 2021 Aug 23;1:24. doi: 10.1038/s43856-021-00025-z

Fig. 4. qRT-PCR of SARS-CoV-2 infection in COVID-19 autopsy tissues.

Fig. 4

2(∆Ct) for SARS-CoV-2 E gene, N gene, and RdRP gene using RNaseP housekeeping gene as reference. The undetermined Ct was set to 40. Normal lung, H1N1 influenza lung, and normal prostate tissues were used as negative controls. The 2(∆Ct) of normal lung was set as a cutoff point (0.000224) and the gray area below the cutoff line is considered as SARS-CoV-2 negative. Minimum 1 out of the 3 genes should have Ct value < 40 (or 2(∆Ct) > 0.000224) to be considered as SARS-CoV-2 positive.