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. 2001 Jun;45(6):1693–1699. doi: 10.1128/AAC.45.6.1693-1699.2001

FIG. 1.

FIG. 1

PBP profiles of AMP-susceptible and BLNAR H. influenzae strains (A) and Rd strains transformed with the ftsI gene encoding PBP 3 (B). Membrane fractions were labeled with [3H]PEN for 10 min at 30°C and were then subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis and fluorography (I). PBPs were named according to the proposal of Parr and Bryan (19), our data on PBP affinity, and morphologic observations. AMP MICs are as follows: for strain MT053 (lane 1) and MT196 (lane 2), 0.125 μg/ml; for strain MT066 (lane 3), 2 μg/ml; for strain ME870 (lane 4), 4 μg/ml; for strain MT040 (lane 5), 2 μg/ml; for strain ME1133 (lane 6), 4 μg/ml; for strain ME587 (lane 7), 1 μg/ml; for strain KK01 (lane 8), 1 μg/ml; for strain ME495 (lane 9), 1 μg/ml. (B) lane 1, recipient Rd strain of H. influenzae; lane 2, transformed strain Rd3A/ME870-11; lane 3, transformed strain Rd3A/ME870-12; lane 4, BLNAR strain ME870.