TABLE 3.
Assigned group | Straina | MIC (μg/ml)
|
|||||||
---|---|---|---|---|---|---|---|---|---|
AMP | CEC | CTX | CDR | CPD | CDN | CPN | MEM | ||
Rd (ATCC51097) | 0.25 | 1 | 0.008 | 0.25 | 0.063 | 0.008 | 0.016 | 0.031 | |
I | Rd3A/H17 | 1 | 16 | 0.063 | 1 | 0.25 | 0.063 | 0.063 | 0.125 |
II | Rd3A/KK01 | 1 | 16 | 0.063 | 1 | 0.5 | 0.031 | 0.063 | 0.125 |
III | Rd3A/ME870 | 2 | 16 | 0.5 | 2 | 2 | 0.125 | 1 | 0.25 |
H. influenzae Rd (ATCC 51097) (the recipient) was transformed with PCR-amplified ftsI gene DNA fragments from group I, II, and III strains as the donors. Transformants were selected on plates containing 5 or 10 μg of CEC per ml. As an example of the transformant nomenclature, Rd3A/ME870 corresponds to Rd strain containing the ftsI gene from strain ME870.