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. 2022 Apr 19;80(1):ftac009. doi: 10.1093/femspd/ftac009

Figure 1.

Figure 1.

Vascular normalization reduces M. marinum granuloma hypoxia in adult zebrafish. (A) Quantification of average bacterial burden by histological fluorescent pixel count across four paired experiments with at least three animals. Each data point represents the average bacterial fluorescent pixel count for all animals within an experimental replicate. Statistical testing by ratio paired T-test. (B) Representative image of a cluster of hypoxyprobe-positive necrotic granulomas from the testes a 2 wpi M. marinum-infected zebrafish. M. marinum are magenta, hypoxyprobe staining is green, and DAPI staining is blue. White arrow indicates a hypoxyprobe positive granuloma and yellow arrow indicates a hypoxyprobe negative granuloma. Scale bar represents 100 µm. (C) Quantification of granuloma hypoxia by hypoxyprobe staining. Total granulomas analyzed DMSO = 27 and AKB-9785 = 35; total animals analyzed: DMSO = 3 and AKB-9785 = 3. Statistical testing by Fisher's exact test on raw counts.